Eurospher II 100-10 biphenyl column 300 x 4.6 mm
High-purity, high-performance HPLC phase based on fully porous silica particles with phenyl-hexyl modification for both, hydrophobic and aromatic ππ interactions.
Ultra pure, spherical high performance HPLC phase based on silica gel
The biphenyl phase is an advanced aromatic reverse phase with two conjugated phenyl rings for various separation mechanisms.
Properties
Separation mechanism: weakly hydrophobic, more polar and intensive aromatic ππ interactions. Reversed phase, normal phase, cation exchange or HILIC mode are possible.
Key features
Particularly sensitive to electron density and substitution patterns in aromatic molecules. Improved separation of structurally similar aromatic isomers.
High selectivity toward conjugated double bonds.
Stable retention in highly aqueous mobile phases even with 100% water.
Recommended application areas
Suitable for acidic, basic, neutral and aromatic analytes e.g. benzene derivatives, PAHs, PAKs, positional isomers (ortho/meta/para), polar analytes with conjugated double bonds
Internal Reference:
30EE091E2N
Technical Data
| General | |
|---|---|
| Brand | Eurospher II |
| Units per Package | 1 |
| Includes | Column |
| Separation Mode | RP (Reversed Phase), NP (Normal Phase), IEX (Cation Exchange), HILIC (Hidrophylic Interaction Liquid Chromatography) |
| Modification | Biphenyl |
| Endcapping | Double |
| Capillary & Tubing Connection | 1/16" |
| USP Code | L11 |
| Specifications | |
|---|---|
| Column Length | 300 mm |
| Column Inner Diameter | 4.6 mm |
| Column Particle Size | 10 µm |
| Column Pore Size | 100 Å |
| pH Range | 2-8 |
| Carbon Load | 14 % |
| Surface Area | 350 m²/g |
| Max. Pressure | 400 bar |
| Temperature Range | 50 °C |
| GMP Relevant | |
|---|---|
| Housing Material | Stainless steel |
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