AppliChrom VivoSep SEC 400, 300 x 20 mm SEC column, 10 µm, Separation range 10 - 5 000 kDa
High quality hydrophilic SEC column with extended pressure stability for the separation of proteins, peptides and amino acids.
Multi hydrophilic layered SEC media for highest efficiency and low interaction
Properties
Available seperation ranges from small molecules up to 5 500 kDa.
Stable between pH 2–10 and up to 90 °C for various SEC applications.
Key features
VivoSep columns enable simple adaptation from HPLC to SEC with stainless steel instruments. Thus, they are a versatile tool for accurate protein molecular weight or size determination.
Low column bleed for low detector noise
Increased lifetime of SEC columns through combination of proprietary particle and packing technology
Recommended eluents
H₂O, buffers
Application examples
Water soluble proteins, humic substances
Internal Reference:
30JT46BABN
Technical Data
| General | |
|---|---|
| Brand | AppliChrom® VivoSep |
| Units per Package | 1 |
| Includes | Column |
| Column Separation Mode | SEC (Size Exclusion) |
| Column Length | 300 mm |
| Column Inner Diameter | 20 mm |
| Column Particle Size | 10 µm |
| Specifications | |
|---|---|
| Column Base Material | Multi hydrophilic layered SEC media for highest efficiency and no silanol interactions |
| Modification | SEC 400 |
| Column Separation Range | 10 - 5 000 kDa |
| Capillary Connection | 1/16" |
| Housing Material | Stainless Steel (SST) |
| pH Range | 2-11 |
| Maximum Pressure | 200 bar |
| Temperature Range | 10-90°C |
| Eluent Recommendation | Buffer, water (with salt additives) |
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