AppliChrom VivoSep SEC 300, 50 x 8 mm SEC column, 300 Å, 7 µm, Separation range 1000-300 000 Da
Hydrophilic multistage crosslinked polymeric SEC material with 200 bar pressure and increased pH stability. Perfect to determine proteins or antibodies.
Multi hydrophilic layered SEC media for highest efficiency and low interaction
Properties
Available seperation ranges from small molecules up to 5 500 kDa.
Stable between pH 2–10 and up to 90 °C for various SEC applications.
Key features
VivoSep columns enable simple adaptation from HPLC to SEC with stainless steel instruments. Thus, they are a versatile tool for accurate protein molecular weight or size determination.
Low column bleed for low detector noise
Increased lifetime of SEC columns through combination of proprietary particle and packing technology
Recommended eluents
H₂O, buffers
Application examples
Water soluble proteins, humic substances
Internal Reference:
05GK46BABL
Technical Data
| General | |
|---|---|
| Brand | AppliChrom® VivoSep |
| Units per Package | 1 |
| Includes | Column |
| Column Separation Mode | SEC (Size Exclusion) |
| Column Length | 50 mm |
| Column Inner Diameter | 8 mm |
| Column Particle Size | 7 µm |
| Column Pore Size | 300 Å |
| Specifications | |
|---|---|
| Column Base Material | Multi hydrophilic layered SEC media for highest efficiency and no silanol interactions |
| Modification | SEC 300 |
| Column Separation Range | 1 - 300 kDa |
| Endcapping | No |
| Capillary Connection | 1/16" |
| Housing Material | Stainless Steel (SST) |
| pH Range | 2-11 |
| Maximum Pressure | 200 bar |
| Temperature Range | 10-90°C |
| Eluent Recommendation | Buffer, water (with salt additives) |
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